PolyethyleneGlycolCan Be ValidlyOmittedfromChromogenicPeptide
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چکیده
Protein concentration in patients’ urine ranges from almost null to about 60 g/L. Many substances in urine can interfere with the reaction between protein and reagent. Moreover, many kinds of dye show a strong tendency to stick to glassware, including cuvettes. For these reasons, no really satisfactory colorimetric method has yet been devised for assay of protein in urine, especially in an automated analyzer system.With the present method protein concentrations in urine ranging from 10 to 16000 mg/L can be measured, without dilution of the sample. The reagent does not adsorb onto the wall of cuvettes, so this method is applicable to an automated analyzer of the discrete type. Negative errors due to possible interferents, oxalate and other chelating substances in urine, could be eliminated by prior addition of oxalate to the reagent to give a 1 mmol/L final concentration. The difference in the reactivity for various kinds of protein in this method was compared with that in a biuret method. Any current methods determine the concentration of protein in body fluid by measuring one kind of physicochemical property of protein, so the results obtained by different methods on different bases do not usually agree
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